in whole cell patch clamp experiment, using the XC buffer from our lab will introduce “white precipitation” on the previously bleached reference electrode (AgCl)
however using the buffer from medical school will not have the same effect
potential solutions
- sandpaper sand down the raw silver wire and bleach to make sure a better layer formation?
- testing a non-Ca buffer if the white thing are actually calcium carbonate.
useful links
Why do people use Ag/AgCl electrodes for a patch-clamp? | ResearchGate VHLab Wiki