Sreeja Sasidharan1, Leah Knepper2, Emily Ankrom2, Gabriel Cucé1, Amanda Ratajczak1, Damien Thévenin2, and Aurelia Honerkamp-Smith1,* 1Department of Physics, Lehigh University, 16 Memorial Drive E, Bethlehem, PA 2Department of Chemistry, Lehigh University, 6 E. Packer Avenue, Bethlehem, PA *Correspondence: auh216@lehigh.edu
-
Paulick, M. G., and C. R. Bertozzi, 2008. The Glycosylphosphatidylinositol Anchor: A Complex Membrane-Anchoring Structure for Proteins. Biochemistry 47:6991–7000. https://pubs.acs.org/doi/10.1021/bi8006324.
-
Fujihara, Y., and M. Ikawa, 2016. GPI-AP release in cellular, developmental, and reproductive biology. Journal of Lipid Research 57:538–545. https://linkinghub.elsevier.com/retrieve/pii/S0022227520354109.
-
Banerjee, T., S. Matsuoka, D. Biswas, Y. Miao, D. S. Pal, Y. Kamimura, M. Ueda, P. N. Devreotes, and P. A. Iglesias,
-
A dynamic partitioning mechanism polarizes membrane protein distribution. Nature Communications 14:7909. https://www.nature.com/articles/s41467-023-43615-2.
-
Ebong, E. E., F. P. Macaluso, D. C. Spray, and J. M. Tarbell, 2011. Imaging the Endothelial Glycocalyx In Vitro by Rapid Freezing/Freeze Substitution Transmission Electron Microscopy. Arteriosclerosis, Thrombosis, and Vascular Biology 31:1908–1915.
-
Van Den Berg, B. M., J. A. E. Spaan, and H. Vink, 2009. Impaired glycocalyx barrier properties contribute to enhanced intimal low-density lipoprotein accumulation at the carotid artery bifurcation in mice. Pflügers Archiv - European Journal of Physiology 457:1199–1206. http://link.springer.com/10.1007/s00424-008-0590-6.
-
Dai, G., M. R. Kaazempur-Mofrad, S. Natarajan, Y. Zhang, S. Vaughn, B. R. Blackman, and R. D. Kamm, 2004. Distinct endothelial phenotypes evoked by arterial waveforms derived from atherosclerosis-susceptible and -resistant regions of human vasculature. Proceedings of the National Academy of Sciences 101:14871–14876.
-
Beurskens, D. M., M. E. Bol, T. Delhaas, M. C. Van De Poll, C. P. Reutelingsperger, G. A. Nicolaes, and J.-W. E. Sels,
-
Decreased endothelial glycocalyx thickness is an early predictor of mortality in sepsis. Anaesthesia and Intensive Care 48:221–228. http://journals.sagepub.com/doi/10.1177/0310057X20916471.
-
Dong, C., Y. K. Choi, J. Lee, X. F. Zhang, A. Honerkamp-Smith, G. Widmalm, L. J. Lowe-Krentz, and W. Im, 2021. Structure, Dynamics, and Interactions of GPI-Anchored Human Glypican-1 with Heparan Sulfates in a Membrane. Glycobiology 31:593–602. https://academic.oup.com/glycob/article/31/5/593/5918341.
-
Ohi, M. D., and A. K. Kenworthy, 2022. Emerging Insights into the Molecular Architecture of Caveolin-1. Journal of Membrane Biology 255:375–383.
-
Tao, X., C. Zhao, and R. MacKinnon, 2023. Membrane protein isolation and structure determination in cell-derived membrane vesicles. Proceedings of the National Academy of Sciences 120:e2302325120. https://pnas.org/doi/10. 1073/pnas.2302325120.
-
Groves, J., and S. Boxer, 1995. Electric field-induced concentration gradients in planar supported bilayers. Biophysical Journal 69:1972–1975. https://linkinghub.elsevier.com/retrieve/pii/S0006349595800676.
-
Liu, C., C. F. Monson, T. Yang, H. Pace, and P. S. Cremer, 2011. Protein Separation by Electrophoretic–Electroosmotic Focusing on Supported Lipid Bilayers. Analytical Chemistry 83:7876–7880. https://pubs.acs.org/doi/10.1021/ ac201768k.
-
Huang, S.-H., B.-C. Huang, and L. Chao, 2022. Development of Cell Membrane Electrophoresis to Measure the Diffusivity of a Native Transmembrane Protein. Analytical Chemistry 94:4531–4537. https://pubs.acs.org/doi/10.1021/ acs.analchem.2c00211.
-
Engstler, M., T. Pfohl, S. Herminghaus, M. Boshart, G. Wiegertjes, N. Heddergott, and P. Overath, 2007. Hydrodynamic Flow-Mediated Protein Sorting on the Cell Surface of Trypanosomes. Cell 131:505–515. https://linkinghub. elsevier.com/retrieve/pii/S0092867407011440.
-
Jönsson, P., A. Gunnarsson, and F. Höök, 2011. Accumulation and Separation of Membrane-Bound Proteins Using Hydrodynamic Forces. Analytical Chemistry 83:604–611.
-
Jönsson, P., J. McColl, R. W. Clarke, V. P. Ostanin, B. Jönsson, and D. Klenerman, 2012. Hydrodynamic trapping of molecules in lipid bilayers. Proceedings of the National Academy of Sciences 109:10328–10333. https://www.pnas. org/doi/full/10.1073/pnas.1202858109, publisher: Proceedings of the National Academy of Sciences.
-
Hu, S.-K., L.-T. Huang, and L. Chao, 2016. Membrane species mobility under in-lipid-membrane forced convection. Soft Matter 12:6954–6963. http://xlink.rsc.org/?DOI=C6SM01145D.
-
Ratajczak, A. M., S. Sasidharan, X. I. R. Gonzalez, E. J. Miller, L. Socrier, A. A. Anthony, and A. R. Honerkamp-Smith,
-
Measuring flow-mediated protein drift across stationary supported lipid bilayers. Biophysical Journal 122:1720–1731.
-
Lim, K. H., H. Huang, A. Pralle, and S. Park, 2013. Stable, high-affinity streptavidin monomer for protein labeling and monovalent biotin detection. Biotechnology and Bioengineering 110:57–67. https://onlinelibrary.wiley.com/ doi/10.1002/bit.24605.
-
Lim, K. H., H. Huang, A. Pralle, and S. Park, 2011. Engineered Streptavidin Monomer and Dimer with Improved Stability and Function. Biochemistry 50:8682–8691. https://pubs.acs.org/doi/10.1021/bi2010366.
-
Angelova, M. I., S. Soléau, P. Méléard, F. Faucon, and P. Bothorel, 1992. Preparation of giant vesicles by external AC electric fields. Kinetics and applications, Steinkopff, Darmstadt, 127–131. Progress in Colloid & Polymer Science.
-
Seelig, J., 1990. Interaction of phospholipids with Ca2+ ions. On the role of the phospholipid head groups. Cell Biol Int Rep 14:353–360.
-
Chada, N., K. P. Sigdel, R. R. S. Gari, T. R. Matin, L. L. Randall, and G. M. King, 2015. Glass is a Viable Substrate for Precision Force Microscopy of Membrane Proteins. Sci Rep 5:12550.
-
Galush, W. J., J. A. Nye, and J. T. Groves, 2008. Quantitative Fluorescence Microscopy Using Supported Lipid Bilayer Standards. Biophysical Journal 95:2512–2519.
-
Jönsson, P., J. P. Beech, J. O. Tegenfeldt, and F. Höök, 2009. Mechanical Behavior of a Supported Lipid Bilayer under External Shear Forces. Langmuir 25:6279–6286.
-
Zhang, H.-Y., and R. J. Hill, 2010. Lipopolymer gradient diffusion in supported bilayer membranes. Journal of The Royal Society Interface 8:312–321.
-
Miller, E. J., M. D. Phan, J. Shah, and A. R. Honerkamp-Smith, 2023. Passive and reversible area regulation of supported lipid bilayers in response to fluid flow. Biophysical Journal 122:2242–2255. https://www.sciencedirect.com/ science/article/pii/S0006349523000280.
-
Gurdap, C. O., L.Wedemann, T. Sych, and E. Sezgin, 2022. Influence of the extracellular domain size on the dynamic behavior of membrane proteins. Biophysical Journal 121:3826–3836. https://linkinghub.elsevier.com/retrieve/pii/ S0006349522007603.
-
Liao, Y.-H., C.-H. Lin, C.-Y. Cheng, W. C. Wong, J.-Y. Juo, and C.-L. Hsieh, 2019. Monovalent and Oriented Labeling of Gold Nanoprobes for the High-Resolution Tracking of a Single-Membrane Molecule. ACS Nano 13:10918–10928. https://pubs.acs.org/doi/10.1021/acsnano.9b01176.
-
Jönsson, P., and B. Jönsson, 2015. Hydrodynamic Forces on Macromolecules Protruding from Lipid Bilayers Due to External Liquid Flows. Langmuir 31:12708–12718.
-
Chaoui, M., and F. Feuillebois, 2003. Creeping flow around a sphere in a shear flow close to a wall. The Quarterly Journal of Mechanics and Applied Mathematics 56:381–410.
-
Erickson, H. P., 2009. Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy. Biological Procedures Online 11:32–51.
-
Fleming, P. J., and K. G. Fleming, 2018. HullRad: Fast Calculations of Folded and Disordered Protein and Nucleic Acid Hydrodynamic Properties. Biophysical Journal 114:856–869. https://linkinghub.elsevier.com/retrieve/pii/ S0006349518300651.
-
Camley, B. A., and F. L. H. Brown, 2019. Motion of objects embedded in lipid bilayer membranes: Advection and effective viscosity. Journal of Chemical Physics 151:124104. http://aip.scitation.org/doi/10.1063/1.5121418.
-
Knight, J. D., M. G. Lerner, J. G. Marcano-Velázquez, R. W. Pastor, and J. J. Falke, 2010. Single Molecule Diffusion of Membrane-Bound Proteins: Window into Lipid Contacts and Bilayer Dynamics. Biophysical Journal 99:2879–2887. https://linkinghub.elsevier.com/retrieve/pii/S0006349510010428.
-
Tanaka, K., D. Joshi, S. Timalsina, and M. A. Schwartz, 2021. Early events in endothelial flow sensing. Cytoskeleton 78:217–231. https://onlinelibrary.wiley.com/doi/10.1002/cm.21652.